How are Salmonella serotypes typically identified?

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Salmonella serotypes are predominantly identified based on their O antigen, which is a part of the lipopolysaccharide layer of the bacterial cell wall. The presence of specific O antigens distinguishes different serotypes of Salmonella. O antigens are essential for serotyping because they elicit a strong immune response and are highly variable between serotypes.

While H antigens, which correspond to the flagellar proteins, can also be valuable for serotyping certain Salmonella species, the O antigen is the primary basis for classification and identification of the various serotypes. This is due to the fact that the O antigens are more stable and provide consistent marker differences among the numerous serotypes.

K antigens, which are capsular antigens, are less commonly used for initial identification compared to O and H antigens. Lastly, the DNase test is not a relevant method for identifying Salmonella serotypes; it is primarily used in differentiating bacterial species based on their ability to degrade DNA.

Thus, the emphasis on the O antigen underlies its critical role in the serotype determination process for Salmonella, making it the correct choice for identification.

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