What does an oxidase test indicate about a microorganism?

Prepare for the ASCP Technologist in Microbiology (M) Exam. Access interactive quizzes with hints and explanations. Enhance your knowledge and get ready to ace your certification!

The oxidase test is specifically designed to detect the presence of cytochrome c oxidase, which is an enzyme involved in the electron transport chain of bacteria. When a microbe possesses this enzyme, it can catalyze the oxidation of certain substrates, leading to a color change in the test reagent. This indicates that the microorganism can utilize oxygen as a terminal electron acceptor in its respiratory process.

In contrast, the other options relate to different metabolic capabilities or characteristics of microorganisms. The ability to utilize lactose pertains to lactose fermentation, which is not assessed by the oxidase test. The production of hydrogen sulfide involves different biochemical pathways and is typically tested using media like Triple Sugar Iron (TSI) agar. Resistance to antibiotics is determined through other methodologies such as sensitivity testing, which does not involve the oxidase reaction. Thus, the primary significance of the oxidase test is specifically tied to the detection of cytochrome c oxidase activity.

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